Aflatoxin Biodetoxification Strategies Based on Postbiotics.

Aflatoxins (AFs) are secondary metabolites produced by fungi, and they are deemed the most perilous mycotoxin and food safety predicament. The exposure of humans to mycotoxins transpires either directly through the consumption of contaminated agricultural commodities or indirectly through the ingestion of items derived from animals that have been nourished with tainted substances of animal origin. To ensure the detoxification of AFs in animal and plant food products and to mitigate the risks they pose to public health and the economy, diverse techniques (physical, chemical, and biological) have been subject to scrutiny. By altering and eradicating the molecular structure of the toxin, all of these approaches impede its transmission to the digestive system and potentially diminish the accessibility of toxins to the target tissue, ultimately eliminating them. Given the pervasive predicaments attributed to the contamination of foods and feeds by AFs, it is of utmost importance to urgently devise cost-effective and appropriate strategies to combat this hazard. This review highlights the concept of AFs, definitions, and benefits of postbiotics and their biological role in the detoxification of AFs, as well as their benefits in the food-pharmaceutical industry.

L-theanine combination therapy with fluvoxamine in moderate-to-severe obsessive-compulsive disorder: A placebo-controlled, double-blind, randomized trial.

AIM: The main aim of this study was to investigate the additional effects of L-theanine, an amino acid in tea and an analog of glutamate with neuroprotective and anti-depressant properties, on obsessive-compulsive disorder (OCD) symptoms in combination with fluvoxamine. METHODS: Patients from either sex aged between 18 and 60 years diagnosed with OCD, based on the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5), who had a Yale-Brown Obsessive Compulsive Scale (Y-BOCS) score of more than 21 were enrolled in a double-blinded, parallel-group, placebo-controlled, clinical trial of 10 weeks to receive either L-theanine (100 mg twice daily) and fluvoxamine (100 mg daily initially followed by 200 mg daily after week 5) or placebo and fluvoxamine. The primary outcome of interest in this study was the Y-BOCS total score decrease from baseline. RESULTS: From a total of 95 evaluated patients, 50 completed our study; 30 were randomly assigned to each group. Multivariate analysis (ANOVA) showed a significant effect of time × $$ \times $$ treatment for L-theanine in obsession subscale (F = 5.51, P = 0.008) of the Y-BOCS score but not in the total and compulsion scores. Our results showed significantly more improvement in obsession subscale scores in L-theanine compared to placebo group (P = 0.007, Cohen's d = 0.82). Also, total Y-BOCS scores were lower in L-theanine compared to placebo group at week 5 (P = 0.039, Cohen's d = 0.60) and 10 (P = 0.008, Cohen's d = 0.80). However, there was no significant between-group differences in compulsion subscale scores. Complete response was also more frequent in the L-theanine group (P = 0.0001). CONCLUSION: Findings in this study suggest L-theanine as a relatively safe and effective adjuvant therapy for moderate to severe OCD.

Calcitriol combined with fish oil in the diet of Chukar partridge can ameliorate laying performance by improving oviduct histomorphometry and functions.

The effects of calcitriol (CT) and/or fish oil (FO) on performance, oviposition time, sex ratio and morphology of the reproductive system of laying Chukar partridges were studied. Female (n = 48) and male (n = 16) partridges were used in a completely randomised design using a 2 × 2 factorial arrangement and were randomly allocated to either of four experimental treatments with four cage replicates of three females and one male each. Female birds received no FO (CON - FO) or were orally administered with 0.2 mL (0.24 g)/500 g body weight FO (CON + FO) or 0.2 mL solution containing 10 µg CT (CT - FO), or their combination (CT + FO) for 42 successive days. The eggs were collected every two hours between 07:00 and 23:00 h. Administering FO along with CT had considerably increasing effect on the male-biased sex ratio. FO and CT administration interacted to increase serum calcium concentration. Experimental treatments increased the number of leucocytes and erythrocytes. Serum cholesterol was decreased in CON + FO partridges compared with those of the CT - FO and CON - FO birds. There was an interaction between FO and CT on the weight of eggs and hatchlings, number of medium white follicles, diameter and the number of small yellow follicles, the weight and diameter of the fifth follicles (F5), and thickness of secondary mucosal folds in both uterus and vagina. Administering CT alone or with FO increased the feed intake, egg production, oviductal weight, diameter and number of large yellow follicles, the weight and diameter of the second (F2) and first (F1) follicles compared with those of the CON - FO females. Further studies are required to elucidate the mechanisms by which such changes in the sex ratio skew, ovary and oviduct are mediated.

In vitro rumen fermentation pattern: insights from concentrate level and plant oil supplement.

The addition of oil to ruminant diets and oil fatty acid profiles are major factors that negatively affect ruminal fermentation, while increased forage level attenuates the adverse effects. The aim of this study was to determine the effects of oil source supplementation and concentrate level in the diet on in vitro ruminal fermentation kinetics. Pomegranate, garlic or sunflower oils were added (2 % dry matter (DM) basis) to the diets containing 40 % or 60 % (DM basis) concentrates. In vitro gas production parameters, pH, ammonia nitrogen concentration and total protozoa count were measured. Additionally, metabolizable energy (ME), short-chain fatty acid (SCFA) production and organic matter digestibility (OMD) were determined. Rumen fermentation parameters and protozoal population counts were analyzed as a completely randomized design with a 2  × â€¯3 factorial arrangement of treatments, and gas production parameters were analyzed as a 2  × â€¯3 factorial arrangement in a randomized block design. The results showed that the HCPO (high (60 %) concentrate diet containing pomegranate oil) and HCSO (high (60 %) concentrate diet containing sunflower oil) diets produced the highest (5.40 mg dL - 1 ) and lowest (2.61 mg dL - 1 ) concentrations of NH 3 -N ( p > 0.01 ), respectively. Total protozoa count tended ( p = 0.07 ) to be highest in HCPO and lowest in HCSO diets (5.10 vs. 4.81 Log 10 g - 1 digesta). No interaction effects between the concentrate level and oil source were found on in vitro gas production parameters, pH, estimated ME, SCFA and OMD, and Entodinium and Diplodinium populations ( p > 0.05 ). It is concluded that dietary supplementation with highly unsaturated oil from three different sources at 2 % level (DM basis) had no apparent effects on in vitro ruminal fermentation patterns.

Reproductive performance and progeny sex ratio of female Chukar (Alectoris chukar) breeder partridges reared on restricted feeding regimens.

A hypothesis was tested that quantitative feed restriction affects the reproductive performance and offspring sex ratio of female Chukar breeder partridges. A total of 160 2.5-year-old male and female partridges were randomly allotted to four treatment groups. The birds in the control group were fed ad libitum, whereas those in treatments G95 , G90 , and G85 received 26.1, 24.7, and 23.3 g of feed per bird/day to provide 95%, 90%, and 85% of ad libitum feeding level, respectively. The reproductive performances of female Chukar partridges including egg production, egg quality, fertility rate, duration of fertility, hatchability, chick quality, mortality rate, and offspring sex ratio (using a PCR procedure) were investigated. Feed restriction of all levels decreased the body weight and egg production compared with the ad libitum birds; however, restricted feeding had no significant effect on the egg quality traits. Non-significant effects of treatment on fertility and hatchability rate were found. The restricted feeding reduced the duration of fertility. Furthermore, maternal restricted feeding resulted in decreased chick weights. The results of this study showed that embryonic mortality was not affected by the restricted feeding of Chukar breeder partridges. Interestingly, restricted feeding was associated with a decreased proportion of male offspring. Overall, body weight, egg production, duration of fertility, progeny chick weight, and sex ratio were responsive to restricted feeding where their changes make the restricted feeding regimens to not be practically recommended in breeder partridge production. These results are in contrast to the conventional restricted feeding program implemented in broiler breeder industry.

Alarming impact of the excessive use of tert-butylhydroquinone in food products: A narrative review.

Tert-butyl hydroquinone (TBHQ) is a food additive commonly used as a more effective protectant in the food, cosmetic and pharmaceutical industries. However, the long-term exposure to TBHQ at higher doses (0.7 mg/kg) results in substantial danger to public health and brings a series of side effects, including cytotoxic, genotoxic, carcinogenic, and mutagenic effects. As a result, the global burden of chronic diseases has fascinated consumers and governments regarding the safety assessment of food additives. Regarding contradictory reports of various research about the application of food additives, the accurate monitoring of food additives is urgent. Notwithstanding, there are reports of the therapeutic effects of TBHQ under pathologic conditions through activation of nuclear factor erythroid 2-related factor 2. Thus, further investigations are required to investigate the impact of TBHQ on public health and evaluate its mechanism of action on various organs and cells. Therefore, this review aimed to investigate TBHQ safety through an overview of its impacts on different tissues, cells, and biological macromolecules as well as its therapeutic effects under pathologic conditions.

Medication Adherence and Drug Attitude Amongst Male Patients with the Methamphetamine-Induced Psychotic Disorder After Discharge: A Three Months Follow Up Study.

Medication adherence and attitudes toward psychiatric medication affect treatment outcome. The objective of this study was to evaluate the effect and interaction of psychotic symptoms, sociodemographic factors, and attitudes concerning medication use with a three-month follow-up among methamphetamine-induced psychotic male patients. In this prospective, descriptive study, 42 male patients diagnosed with a methamphetamine-induced psychotic disorder were selected on the last day of their admission period in Iran Psychiatry Hospital, Tehran, Iran. Each patient was evaluated using the Persian version of the Drug Attitude Inventory (DAI-10), Medication Possession Ratio (MPR), Positive and Negative Syndrome Scale (PANSS), as well as a sociodemographic questionnaire immediately, one month and three months after discharge. There was a significant difference in MPR between the first and third months. Moreover, the frequency of patients with a positive attitude toward their medications increased over time. Indeed, all participants stated a positive attitude at the last follow-up based on the DAI-10 cutoff. Based on our findings, medication adherence of male patients with methamphetamine-induced psychotic disorder should be an essential aspect of treatment after discharge from psychiatry inpatient wards, more specifically, through the first months.

Reproductive and embryological toxicity of lead acetate in male mice and their offspring and mitigation effects of quercetin.

Exposure to heavy metals not only impacts on fertility in males, it may also affect the offspring. The aim of the present study was to examine the toxic effects of lead acetate on fertility in male mice and their offspring, and the potential effect of quercetin on mitigating the likely effects. Experimental mice were randomly divided into three groups and administered with (i) distilled water (control); (ii) lead acetate (150 mg/kg BW/day); (iii) lead acetate (150 mg/kg BW/day) with quercetin (75 mg/kg BW/day). Lead acetate administration in male mice adversely affected their fertility through changes in sperm motility, viability, morphology, maturity, membrane integrity, and intracellular reactive oxygen species (P <  0.05). Similar findings were observed in the offspring of the lead-treated male mice. Early embryonic development and implantation rate were also adversely influenced in both the sires and offspring when male mice were treated with lead acetate (P <  0.05). The data demonstrated that down-regulation of Cks2 (CDC28 protein kinase regulatory subunit-2) in sperm had an association with early embryonic development in lead acetate treated group. In conclusion, lead acetate administration adversely impacted on the fertility of the male mice and their male offspring fertility; on the other hand, paternal quercetin co-administration somewhat ameliorated the adverse effects of lead on male mice and their offspring.

Detrimental effects of long-term exposure to heavy metals on histology, size and trace elements of testes and sperm parameters in Kermani Sheep.

Exposure to endocrine-disrupting chemicals (EDCs) has been hypothesized as a cause of declining sheep reproductive efficiency. Understanding the long-term effects of EDCs such as heavy metals on reproductive health requires investigation in 'real life' of sheep that are reared in industrial areas. The aim of this study was to evaluate the effect of long-term exposure of Kermani rams to high levels of environmental heavy metals probably emitted from a copper smelter at KhatoonAbad in ShahreBabak, Kerman province. Testicular characteristics were determined in randomly-selected rams (3-4 years old) at 4 directions (south, north, east, and west) and 4 distances (10, 20, 30, and 40 km) from the smelter. Testicular trace element contents, size, serum testosterone, histological attributes and seminal characteristics, except semen volume, were affected by both the direction and the distance from the smelter (P < 0.05). Testicular contents of Pb, Cd, Cr, and Ni, and sperm abnormalities were higher at 10 km south from the smelter and lower at 40 km west. Other parameters were higher at 40 km west and lower at 10 km south. Interestingly, the testicular contents of Cu at 10 km south were lower and associated with higher sperm abnormalities in the rams reared closer to the smelter. The highest weight, length and circumference of the testis were found at 40 km west. The lowest concentration of testosterone was observed at 10 km south, being 92.6% lower than the highest values obtained at 40 km west. The diameter of seminiferous tubules and epithelial height at 10 km south were 8.9% and 27.5% lower than the highest values obtained at 40 km west. A positive correlation between Pb, Cd, Cr and Ni contents in the testis with sperm abnormalities, and a negative correlation between these elements with the other parameters were found. It was concluded that long-term exposure to heavy metals might have been a cause of decreased fertility in rams and probably other living species in this region.

Beneficial effects of dietary coenzyme Q10 on the productive and reproductive variables of broiler breeder hens.

The aim of this study was to evaluate the effects of supplementary CoQ10 in the diets of aged broiler breeder hens on productive and reproductive variables. A total of 128 hens)44 weeks of age) were randomly assigned to one of 16 groups (eight hens per group). The hen-groups (with equal mean egg production and egg weight) were randomly assigned to one of four diet-groups to provide four pen/groups per treatment. There was no CoQ10 supplementation or supplemental amounts of either 300, 600 or 900 mg CoQ10/kg added to the basal diet. Egg production, weight, and mass were determined weekly. To assess fertility, hatchability, and sperm penetration (SP) rate, the hens were artificially inseminated on a weekly basis (from 47-54 weeks of age). The hens were weighed and killed at the end of the experiment for evaluation of the ovarian morphology, oviduct histology, utero-vaginal junction (UVJ) total antioxidant capacity (TAC), and Pdss2, GDF9, and BMP15 mRNA transcript abundances in the germinal disc regions. The results indicated that there was a linear response curve to increasing amounts of supplemental dietary CoQ10 on fertility, hatchability of eggs, SP rates, TAC of the UVJ, fold height and surface epithelia of the magnum and isthmus, and abundance of GDF9, BMP15 and Pdss2 mRNA transcripts in the germinal disc region. In conclusion, the findings of the present study indicate diet supplementation with CoQ10 had beneficial effects on the productive and reproductive variables of aged hens.

Use of supplemental dietary coenzyme Q10 to improve testicular function and fertilization capacity in aged broiler breeder roosters.

In numerous studies it has been suggested that targeting mitochondria with specific compounds could efficiently inhibit various conditions associated with oxidative stress. The treatment of aged roosters with compounds such as coenzyme Q10 (CoQ10), may improve their reproductive performance by providing protection from oxidative stress. Therefore, this study was performed to assess the effect of supplemental dietary CoQ10 on the testicular function and fertility of aged broiler breeder roosters. A total of 36 roosters)47 weeks of age) were randomly divided into dietary treatments containing either 0, 300 or 600 mg CoQ10/kg diet. Three birds were allocated to each of four replicate groups in each dietary treatment. Between 47 and 54 weeks of age, ejaculates were obtained weekly from the three roosters in each replicate group. Samples in a replicate were pooled and analyzed as a single sample. Between 51 and 54 weeks of age, seminal plasma total antioxidant capacity (TAC), alanine amino transferase (ALAT) and aspartate amino transferase (ASAT) levels were assessed. Fertility, hatchability, and sperm penetration (SP) rates were likewise evaluated. Seminal volume, sperm concentration, sperm plasma membrane functionality, sperm plasma membrane integrity, seminiferous tubule diameter and seminiferous epithelium thickness exhibited quadratic increases in response to increasing levels of dietary CoQ10. Respectively, the 429.19, 433.33, 464.50, 613.50, 392.78 and 447.99 mg/kg dietary concentrations of CoQ10 provided the best results for each of the aforementioned variables. Also, other seminal traits, as well as testosterone concentration, fertility, and SP rates, displayed linear increases in response to the increasing levels of CoQ10. Dietary supplementation of CoQ10 linearly decreased seminal plasma ALAT and ASAT and linearly increased seminal plasma TAC. In conclusion, CoQ10 supplementation in the diet (a minimum of 300 mg CoQ10/kg diet) has the potential to improve the reproductive performance of aged broiler breeder roosters.

Dietary Fish Oil and Vitamin E Reduce Cryoinjuries and Apoptosis in Vitrified Laying Hens' Ovarian Tissue.

The occurrence of cryoinjuries during ovarian tissue freezing necessitates development of methods that can overcome these challenges in cell and tissue cryopreservation. It has been hypothesized that omega-3 and vitamin E diet supplementation to the animal could be an appropriate strategy to preserve post-thaw ovarian quality. The laying hen is an appropriate animal model that can be used to study the effects of providing dietary supplements on the outcomes of ovary cryopreservation. The purpose of the study was to evaluate the dietary effects of fish oil, sunflower oil, and vitamin E on 68 laying hens according to the following treatments: basal diet + 1.5% sunflower oil (control; C); basal diet + 1.5% sunflower oil + 1.1 IU alpha-tocopherol/hen/day (E); basal diet + 1.5% fish oil + 1.1 IU alphatocopherol/hen/day (n-3+E); and basal diet + 1.5% fish oil (n-3). The effects on ovarian structure and preservation, apoptosis-related gene expression, and the fatty acid profiles in ovarian laying hen (n = 7 in each group) were studied. The number of intact primordial follicles in n-3+E group was significantly higher than other groups (85% vs. 71%, 72%, and 77% for n-3+E, C, E, and n-3, respectively; p < 0.01). There was a significant reduction in expression of Cas3, as well as Cas8, in n-3 and n-3+E than C and E (p ≤ 0.05). A trend to decrease in Bak (p = 0.089) and Bak/Bcl2 ratio (p = 0.095) in the mRNA was observed in n-3+E. Ovarian eicosapentaenoic acid (C20:5n3) concentration in n-3 was the highest among C, E, and n-3+E (p < 0.01). Docosahexaenoic acid (C22:6 n-3) concentrations in ovaries of the n-3 group were elevated five times more than control. The n-3: n-6 ratio in groups receiving omega-3 (n-3+E and n-3) was higher than other groups (p < 0.01). In conclusion, consumption of dietary omega-3 fatty acids with vitamin E improves the results of vitrification of ovarian tissues in laying hens.

A comparison of temperament and character among psychiatry, internal medicine, and surgery residents at Iran University of Medical Sciences in the academic year 2013-2014.

Background: This study was conducted to compare temperament and character among psychiatry residents, internal medicine, and surgery residents in hospitals affiliated to Iran University of Medical Sciences in the academic year 2013-2014. Methods: This cross sectional analytical study was conducted on 201 residents using the non-probability method of convenience sampling. Cloninger's 125-item Temperament and Character Inventory (TCI) and Maslach's Burnout Inventory (MBI) were distributed among all the residents in their morning session. Once the questionnaires were completed and submitted, the data were analyzed in SPSS 16. The frequency of different personality traits was analyzed among the selected residents. Results: A total of 49.8% of the respondents were female (n=100) and 50.2% were male (n=101). The results of data analysis showed more novelty seeking, reward dependence, and harm avoidance and less persistence (33.93, p=0.006; 9.00, p=0.056; 32.55, p=0.021; and 2.48, p=0.028, respectively) in psychiatry residents than in surgery residents (31.97, 7.87, 30.74, and 3.12 respectively). Reward dependence was more frequent in internal medicine residents than in surgery residents (9.44 and 7.87 respectively, p=0.002). The self-directedness score was lower in psychiatry residents than in internal medicine residents (p=0.761) and higher than in surgery residents (17.96, 18.30, and 17.57 respectively, p=0.824). The cooperativeness and self-transcendence scores were higher in psychiatry residents than in internal medicine (p=0.943, p=0.199, respectively) and surgery residents (p=0.105, p=0.069 respectively). Conclusion: The different dimensions of personality varied between the surgery, internal medicine, and psychiatry residents. Particularly, this study did not show statistically significant differences in dimensions of character (cooperativeness, self-directedness, and self-transcendence) among surgery, internal medicine, and psychiatry residents. The selection of medical specialties based on character traits significantly contributes to more effective treatment of patients and higher satisfaction of the residents.

Fast free of acrylamide clearing tissue (FACT) for clearing, immunolabelling and three-dimensional imaging of partridge tissues.

Fast free of acrylamide clearing tissue (FACT) is a modified sodium dodecyl sulfate-based clearing protocol for the chemical clearing of lipids that completely preserves fluorescent signals in the cleared tissues. The FACT protocol was optimized to image translucent immunostained brain and non-nervous tissues. For this purpose adult male Chukar partridge (Alectoris chukar) was used as a model. After clearing the tissues, 1 or 2 mm-thickness sections of tissues were immunolabeled. The paraventricular nucleus in the hypothalamus (2-mm section) was cleared with FACT, and then was stained with gonadotropin-inhibitory hormone (GnIH) antibody and Hoechst. Simultaneously, immunohistochemical (IHC) staining of cryosectioned brain (30 µm) was done by GnIH-antibody. The FACT protocol and staining of cell nuclei of nine other tissues were done by a z-stack motorized fluorescent microscope. GnIH-immunoreactive neurons were found by FACT and IHC during the breeding season in male partridges. Deep imaging of the kidney, duodenum, jejunum, lung, pancreas, esophagus, skeletal muscle, trachea, and testis were also done. The FACT protocol can be used for the three-dimensional imaging of various tissues and immunostained evaluation of protein markers. RESEARCH HIGHLIGHT: The FACT is a simple and cheap method for whole tissue clearing. The FACT-cleared tissues can be imaged with simple fluorescent microscopes. For the first time, using the FACT, three-dimensional imaging of various tissues was done.

Association of open field behavior with blood and semen characteristics in roosters: an alternative animal model.

INTRODUCTION: The paucity of literature is addressed regarding the correlation between open field as an individual behavioral trait on reproductive capacity in animals. MATERIALS AND METHODS: To address this, Nine-month-old indigenous roosters were housed in individual cages. Each animal was observed twice a week for ten minutes before feeding in an open field apparatus for two weeks (7:00-12:00PM). RESULTS: Interestingly, it was found that rooster's semen characteristics were correlated with their open field behavior. On the other hand, plasma glucose level as a blood attribute was more correlated with semen characteristics. The open field monitoring also revealed that the roosters with the lowest delay to their first pace had the highest sperm forward motility and lower sperm abnormality. The heterophil to lymphocyte ratio (H:L) was found to be low when pace bout and pace numbers were 20 and 35, respectively. The negative correlation between H:L ratio and semen characteristics (live sperm percentage, sperm concentration, and membrane integrity) may be an indication of poor reproductive performance in fearful roosters with higher H:L ratio. CONCLUSIONS: The data suggested a relationship between open field behavior indices and some reproductive parameters in roosters. The results might be applicable for selection of more reproductive animals. Hence, the rooster may also be useful model for similar studies in other species.

Association of open field behavior with blood and semen characteristics in roosters: an alternative animal model / Asociación entre la conducta de gallos en campo abierto con sus características de sangre y de semen: un modelo animal alternativo

Introduction. The paucity of literature is addressed regarding the correlation between open field as an individual behavioral trait on reproductive capacity in animals. Materials and methods. To address this, Nine-month-old indigenous roosters were housed in individual cages. Each animal was observed twice a week for ten minutes before feeding in an open field apparatus for two weeks (7:00-12:00PM). Results. Interestingly, it was found that rooster's semen characteristics were correlated with their open field behavior. On the other hand, plasma glucose level as a blood attribute was more correlated with semen characteristics. The open field monitoring also revealed that the roosters with the lowest delay to their first pace had the highest sperm forward motility and lower sperm abnormality. The heterophil to lymphocyte ratio (H:L) was found to be low when pace bout and pace numbers were 20 and 35, respectively. The negative correlation between H:L ratio and semen characteristics (live sperm percentage, sperm concentration, and membrane integrity) may be an indication of poor reproductive performance in fearful roosters with higher H:L ratio. Conclusions. The data suggested a relationship between open field behavior indices and some reproductive parameters in roosters. The results might be applicable for selection of more reproductive animals. Hence, the rooster may also be useful model for similar studies in other species

Introducción. Existe poca literatura que analice la relación entre el comportamiento individual en animales en campo abierto y su capacidad reproductiva. Material y métodos. Para cubrir esta laguna se trabajó con gallos de 9 meses de edad que fueron encerrados en jaulas individuales. Cada animal se observó 2 veces a la semana, durante 10min, en una zona de campo abierto durante 2 semanas, antes de la alimentación (07:00-12:00 p.m.). Resultados. Curiosamente, se encontró que la característica del semen de los gallos se correlacionaba con su comportamiento en campo abierto. Por otro lado, el nivel de glucosa en plasma, como un atributo de la sangre, se correlacionaba con las características del semen. La monitorización en campo abierto también reveló que los gallos con la menor demora en su primer canto tuvieron la mayor motilidad de los espermatozoides y la menor alteración del esperma. El nivel más bajo de la relación entre heterófilos y linfocitos (H: L) fue encontrado cuando los números de cantos y el ritmo eran 20 y 35, respectivamente. La correlación negativa entre el índice H:L y las características del semen (porcentaje de espermatozoides vivos, concentración de espermatozoides, e integridad de la membrana) puede ser un indicio de mal desempeño reproductivo de gallos con mayor índice H:L. Conclusiones. Los datos indican que existe relación entre los índices de comportamiento en campo abierto y algunos parámetros reproductivos de los gallos. Los resultados podrían ser aplicables para la selección de los animales más reproductivos. El gallo también puede ser útil como un modelo para estudios similares en otras especies

The Content Validity of the Cultural Formulation Interview (CFI).

AIMS: The present study investigates content validity of the open-ended items in Cultural Formulation Interview in the Diagnostic and Statistical Manual of Mental Disorders, 5th Edition (DSM-5). METHODS: After translating into Persian and assessing the content validity of the items and their modification by psychiatrists, the questionnaire was translated back into English by two translators and then retranslated into Persian. The final Persian version and its back-translation were submitted for approval to the DSM-5 design workshop in the United States. After obtaining the group's approval on the back-translation and permission to use the questionnaire, samples were distributed among panel members and the content validity of the questionnaire was thus examined. RESULTS: The content validity index (CVI) of cultural formulation interview was 0.51 and all the items were acceptable although some, especially those on the cultural perception of the context and the cultural factors affecting current help-seeking, had lower content validity ratios (CVR). CONCLUSION: The cultural formulation interview seems to have an overall acceptable content validity although it is weak and thus needs further studies in relation to the two domains of the cultural perception of the context and the cultural factors affecting help-seeking in the Iranian population.

Ethylene Glycol and Dimethyl Sulfoxide Combination Reduces Cryoinjuries and Apoptotic Gene Expression in Vitrified Laying Hen Ovary.

Successful cryopreservation of avian gonads is important not only for avian breeding but is also crucial for preservation of species, especially of endangered birds. The aim of this study was to evaluate the effect of vitrification by several cryoprotectants on the ovarian tissues of laying hens. Ovarian tissues were randomly divided into six groups: control (nonvitrified: C), dehydrated using ethylene glycol (EG), dehydrated with propylene glycol (PROH), dehydrated using dimethyl sulfoxide (DMSO), and two combined groups, EG+DMSO and EG+PROH. The composition of vitrification solutions was as follows: EG group: V1 = 7.5% EG and V2 = 15% EG +0.5 M sucrose, DMSO group: V1 = 7.5% DMSO and V2 = 15% DMSO +0.5 M sucrose, PROH group: V1 = 7.5% PROH and V2 = 15% PROH +0.5 M sucrose, EG+DMSO group: V1 = 7.5% EG +7.5% DMSO and V2 = 15% EG +15% DMSO +0.5 M sucrose and EG+PROH group: V1 = 7.5% EG +7.5% PROH and V2 = 15% EG +15% PROH +0.5 M sucrose. Ovarian tissues of each group were dehydrated for 10 minutes with V1 solution and 2 minutes with V2. Among the vitrified groups, intact primordial and primary follicles showed significant increase in EG+DMSO, but follicular attrition had the highest rate in the PROH group (p < 0.05). Immunohistochemical analysis showed that the percentage of active caspase 3-positive cells was lower (p < 0.05) when using EG+DMSO versus PROH. Further gene expression of caspase 3, 8, and 9 was highest in the PROH group (p < 0.05). Vitrification of ovaries of laying hens using EG+DMSO can afford effective protection of primordial and primary follicles during preservation and may therefore be successfully used for storing avian gonadal tissues.